Walaa Najm Abood*
Department of Microbiology, University of Diyala, Diyala, IraqFulltext PDF
This research performed to determine the potential protective effects of Tinosporacrispa stems to the rat gastric mucosal injury of induced by ethanol. As well as clarify the role of gastrin, pepsin, Prostaglandin E2 (PGE2), Superoxide Dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA) and cytokines (TGFB1 and TNF-α). Seven groups of rats were orally pre-treated with Tween 20 as vehicle control group, Tween 20 as ulcer group, 20 mg/kg of omeprazole as reference drug group, 100 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kg of extract as the experimental groups. An hour later, induction ulcer by given 95% ethanol orally except vehicle control group. The results have been showed significant ulcer protective effects by reduction the ulcer area and increase the ulcer inhibition grossly and histology. As well as significant elevate the gastric juice pH and increasing the production of mucus. In addition, significant elevated of inflammatory mediators PGE2, increased the activity of SOD and CAT have shown in gastric mucosa and significant elevated of in TGFB1. On the other hand, observed reduction the serum level of gastrin and pepsin, and decreasing the level of MDA and TNF-α. In conclusion, our results proof that T. crispa pretreatment has protective effects in ethanol-induced gastric ulcers in rats. Moreover, these results provide evidence that these protective effects of T. crispa by stimulation of some inflammatory mediators as PGE2, gastrin, TGFB1 and TNF-α. Moreover, important antioxidant enzymes such as SOD and CAT which are scavengers of ROS and therefore prevent gastric injury induced by them.
Tinospora crispa; Gastroprotective; Prostaglandin E2; TGFB1; TNF-α; Antioxidants enzyme
Abood WN. Inflammatory Mediator and Antioxidant Role in Gastroprotective of Tinospora crispa against Ethanol Induced Gastric Ulcer. Ann Microbiol Immunol. 2021; 4(1): 1023..