Ann Microbiol Immunol | Volume 2, Issue 2 | Short Communication | Open Access

Engineered Promoter Selectivity of an ECF Sigma Factor

Sébastien Campagne^* and Frédéric HT Allain

Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland

^*Correspondance to: Sébastien Campagne 

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Abstract

In bacteria, promoter melting by alternative sigma factors is achieved by flipping out the base at the position -10. The identity of this base is determined by the sequence of the variable loop L3 of the sigma factor. Here, we solved the solution structure of an engineered Escherichia coli sigmaE region 2 that recognizes an adenine instead of a cytosine in position -10. Our results demonstrate at the atomic level how Extra Cytoplasmic Function (ECF) sigma factors can be rationally engineered to modify their promoter selectivity.

Keywords:

Bacterial transcription initiation; ECF sigma factor; Promoter melting; Promoter specificity

Citation:

Campagne S, Allain FHT. Engineered Promoter Selectivity of an ECF Sigma Factor. Ann Microbiol Immunol. 2019; 2(2): 1016..