Ann Med Med Res | Volume 5, Issue 1 | Research Article | Open Access
Shuhui Huang#, Lu Nie#, Xiaofang Chen, Yongbao Peng and Yanqiu Liu*
Department of Obstetrics and Gynecology, Jiangxi Maternal and Child Health Hospital, China
*Correspondance to: Yanqiu Liu
Fulltext PDFObjective: HSP70 is essentially an intracellular stress protein, but can be released into the extracellular
space through lysosomes, vacuoles and other non-canonical protein transport mechanisms that do
not depend on endoplasmic reticulum-Golgi apparatus, especially with cell apoptosis and necrosis.
In the liquid, it becomes eHSP70, which produces immune responses through various mechanisms.
Moderately increased HSP70 expression can protect cells from external stimuli, and excessive
HSP70 protein expression can stimulate cell death or reduce growth, which can increase maternal or
fetal complications and thus be detrimental to pregnancy, but how does HSP70 expression induce
pathology? The specific mechanism of pregnancy has not been reported. In this study, we analyzed
whether the abnormal increase of extracellular HSP70 (eHSP70) affects intrauterine development
by affecting iNOS and/or eNOS, thereby reducing NO synthesis in related vessels.
Methods: Using in vitro cell co-culture methodology, human choriocarcinoma cell line-JEG-3 cells
were selected to prepare pLV/sh-HSP70 and pLV/HSP70 lentivirus-JEG-3 cell lines, which were
combined with human umbilical vein endothelial cell line. - Co-culture of HUVEC cells, observe the
changes of HUVEC cell biological indicators and related factors, to explore the effect of JEG-3 cells
with different levels of HSP70 on the expression of NO in HUVEC and the changes of cell growth,
apoptosis and cycle, through the cellular molecular level Elucidation of its potential mechanism of
action.
Results: 1. The overexpressed HSP70 in JEG-3 cells and HUVEC cells could inhibit the expression
level of iNOS in the cells, thereby reducing the content of intracellular NO. 2. The abnormal
expression of HSP70 in JEG-3 cells did not change the cell proliferation rate, cell apoptosis and cell
cycle after co-culture with HUVEC cells.
Conclusion: After co-culture of JEG-3 cells with abnormal overexpression of HSP70 and HUVEC
cells, it can inhibit the expression of iNOS in the cells and reduce the content of NO in endothelial
cells, which may lead to the occurrence of pathological pregnancy.
eHSP70; NO; Pregnancy
Huang S, Nie L, Chen X, Peng Y, Liu Y. Effects of Abnormally Expressed HSP70 in Placental Trophoblast Cells on Umbilical Vein Endothelial Cells. Ann Med Medical Res. 2022; 5: 1046..