Ann Med Med Res | Volume 1, Issue 1 | Research Article | Open Access
Albert Nakanishi1*, Thomas Burris2 and Kristine Griffit2
1Department of Pediatrics, Saint Louis University School of Medicine, USA
2Department of Pharmacology and Physiological Sciences, Saint Louis University School of Medicine, USA
*Correspondance to: Albert Nakanishi
Fulltext PDFBackground: Intravenous access may be difficult in young children and drugs or fluids administered subcutaneously may pose advantages in health resource limited environments such as rural clinics, refugee camps or disaster situations. Hypodermoclysis is the subcutaneous (SQ) infusion of fluids; it was the major way of hydrating patients prior to intravenous catheters. The technique is simple, safe and effective in the treatment of mild to moderate dehydration in adults and children. Hyaluronidase is an enzyme and tissue permeability modifier and when administered with fluids subcutaneously aids in the transfer of fluids into the intravascular space. Drugs administered subcutaneously and augmented with hyaluronidase may reveal enhanced systemic absorption.
Objective: We investigated the pharmacokinetics of ampicillin given subcutaneously to mice with and without SQ hyaluronidase.
Methods: Ten-week-old C57Bl6 mice in 3 study groups: 1) Ampicillin (50 mg/kg, 75 mg/kg and 100 mg/kg) w/SQ hyaluronidase (0.1cc), 2) Ampicillin (50 mg/kg, 75 mg/kg and 100 mg/kg) w/ SQ saline, 3) Ampicillin (50 mg/kg, 75 mg/kg and 100 mg/kg) administered IM. Plasma levels were assessed at 30, 60, 120 and 240 min by ELISA.
Results: Ampicillin administered SQ and augmented with hyaluronidase had enhanced absorption. Maximum concentration (Cmax) and Area Under the Curve (AUC) data also revealed improved absorption of ampicillin augmented with hyaluronidase vs IM ampicillin. Metabolism of ampicillin appears to follow first-order kinetics.
Conclusion: Ampicillin administered with hyaluronidase had enhanced absorption and appears to be equivalent to IM administered ampicillin pharmacokinetically.
Nakanishi A, Burris T, Griffit K. ISSN: Enhanced Systemic Absorption of Hyaluronidase Augmented Subcutaneous Administered Ampicillin in a Mouse Model. Ann Med Medical Res. 2018; 1: 1006.