Ann Cytol Histol | Volume 1, Issue 1 | Mini Review | Open Access

Vital Inhibitor-Based Cytochemical Procedure for Proteasome Visualization

Lukasz P. Bialy* and Izabela Mlynarczuk-Bialy

Department of Histology and Embryology, Medical University of Warsaw, Poland

*Correspondance to: Lukasz P. Bialy 

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Abstract

The fluorescent-inhibitors can be used for cytochemistry of enzymes and this technique gives and interesting alternative for classical immunolabeling in both microscopy and flow cytometry. Here we discuss the published data for proteasome inhibitor is BSc2118 that can be easily conjugated with BODI-PY fluorochromes and describe this novel cytochemical procedure. The direct vital pre-fixed inhibitor-based proteasome staining can be efficiently combined with post-fixed classic immunocytochemical detection of other cellular components after ethanol-based fixation. The fluorescent BSc2118 can be used as aggresome marker in aggresome formation with the same effectiveness as FK2 antibody for polyubiquitinated proteins. Fluorochrome-tagged derivates of BSc2118 can also be used for visualization of inhibitor distribution in organs and tissues in animal model. We found this technique as promising as fast and effective for aggresome formation studies.

Keywords:

Fluorochrome; Proteasome; Aggresome

Citation:

Bialy LP, Mlynarczuk-Bialy I. Vital Inhibitor-Based Cytochemical Procedure for Proteasome Visualization. Ann Cytol Histol. 2019; 1(1): 1001.

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