Amole OO1*, Akinyede AA2 and Obanyero DD2
1Department of Pharmacology, Therapeutics and Toxicology, College of Medicine Lagos State University, Nigeria
2Department of Pharmacology, Therapeutics and Toxicology College of Medicine of the University of Lagos, Nigeria
Background: Clerodendrum polycephalum has been traditionally used as a pain killer, management of paralysis, epilepsy and convulsion. Irrespective of its use in Traditionally African Medicine, no data on sub acute toxicity has been described. Objectives: The present study was designed to assess the acute and sub-acute toxicity of Hydroethanolic leaf extract of Clerodendrum polycephalum (HeCP) in rats for 24 h and 28 days respectively. Materials and Methods: Fasting mice (n=5) were subjected to acute toxicity test at doses of HeCP 1000 mg/kg p.o; 2000 mg/kg p.o; 5000 mg/kg p.o. After 24 h, the animals were then observed for behavioral changes, signs of toxicity and mortality. In the sub acute toxicity test (n=10; HeCP 100 mg/kg p.o. 200 mg/kg p.o. 400 mg/kg p.o. daily). Control group received distilled water (0.2 ml) daily. Blood samples were collected for biochemical and hematological analyses as well as serum antioxidant. Tissues were harvested for histopathological analysis. Results: It showed that treatment of mice with 1000 mg/kg, 2000 mg/kg, and 5000 mg/kg of the extract produced no lethality in the mice following acute toxicity testing. Results also showed that the HeCP did not produce any significant (P<0.05) difference in all the hematological parameters when compared to the control. HeCP (200 mg/kg) produced significant decrease (P<0.05) in the level of catalase when compared to the control. At 200 mg/kg of HeCP, there is a significant increase in the level of AST (Aspartate aminotransferase) in comparison to the control. No significant difference was observed in the rest of biochemical parameters. Conclusion: We conclude from the result obtained in this study that there is a wide margin of safety for the use of this plant for long time administration.
Clerodendrum polycephalum; Toxicity; Hematological and biochemical assays; AST
Amole OO, Akinyede AA, Obanyero DD. Toxicity Studies of the Hydroethanolic Leaf Extract of Clerodendrum polycephalum (Lamiaceae) in Rats. Ann Clin Toxicol. 2021; 4(1):1033.