Shaheen SM1* and Mominur Rahman M
Department of Pharmacy, Daffodil International University, BangladeshFulltext PDF
For preliminary assessment of toxicity the brine shrimp lethality assay is deliberated a useful implement. To assessment natural marine products for pharmacological activity before they are used as a general bio-assay it is essential to evaluate the appropriateness of the brine shrimp methods. Citrus limon L (lemon) is an excellent preventative medicine and in the native medication chest it has a wide range of uses. It can assistance weight loss and reduce the risk of heart disease, anemia, kidney stones, digestive issues, and cancer. The current study was aimed to investigate cytotoxic properties of seed extract of Citrus limon L. (lemon) to give an appropriate guide for future investigation. The cytotoxic activity was determined by calculating the LC50 values. The Citrus limon L. (lemon) seed extract shows very good cytotoxic activity against the brine shrimp nauplii. Compared with the LC50 value of standard vincristine sulphate 1.25 mg/mL, the LC50 value of seed extract remained 5.55 mg/mL. However, these outcomes recommend that experimental extracts of Citrus limon L. (lemon) holds cytotoxic activity which sustenance its used in traditional medicine.
Citrus limon; Cytotoxic activity; Brine shrimp; Vincristine sulphate
Shaheen SM, Mominur Rahman M. Assessment of Cytotoxic Activity Using Artemia salina (Brine Shrimp Nauplii) of Citrus limon L (Lemon) Seed Extract. Ann Clin Toxicol. 2019; 2(3): 1023..