Ann Clin Toxicol | Volume 2, Issue 3 | Research Article | Open Access

Induction of Oxidative Stress by Methotrexate in the Testicular Tissue of Swiss Albino Mice

Ojo Olajumoke Omolara

Department of Biochemistry, Ekiti- State University Ado-Ekiti, Nigeria

*Correspondance to: Ojo Olajumoke Omolara 

Fulltext PDF


Methotrexate toxicity has been reported to occur due to the prolonged usage of the drug for desired therapeutic outcome. Testicular dysfunction is one of the health problems associated with free radical induced oxidative stress leading to degeneration of spermatogenesis and other testicular damages. According to reports, increased production of free radicals as well as weakened antioxidant defense system result to oxidative stress. This study therefore was designed to investigate the potential induction of oxidative stress in the testicular tissue of male mice following methotrexate exposure in male Swiss albino mice. In this study, twenty mice of about equal age 4-5 weeks weighing 25 gm were divided into four groups (n=5). The group I served as the control group and received vehicle, group II, III and IV received 1 mg/kg, 3 mg/kg and 5 mg/kg MTX respectively for 21 days via intraperitoneal route. Testicular reproductive hormones, both enzymatic and non enzymatic antioxidant biomarkers were investigated. Reactive Oxygen Species (ROS) and sperm parameters were also evaluated. Marked increase in ROS and LPO levels with simultaneous depletion of CAT, SOD and GSH was seen confirming generation of oxidative stress leading to dysfunction and damage of testicular tissue of the mice treated with Methotrexate.


Methotrexate; ROS; Oxidative stress; Testicular tissue; Hormones


Omolara OO. Induction of Oxidative Stress by Methotrexate in the Testicular Tissue of Swiss Albino Mice. Ann Clin Toxicol. 2019; 2(3): 1022.

Subscribe to Our Newsletter