Houda En-nassiri , Abderrahmane Hammoumi , Thami Berradi , Mohamed Rajaallah , Khadija Essoucratti1 , Rajae Asmai4 , Meriem Khayatti , Brahim Bouchrif and Bouchra Karraouan
Department of Microbiology, Pharmacology, Biotechnology and Environment, Hassan II University of Casablanca, Morocco Labour Regional Laboratory of Analysis and Research (LRARC), Rural Commune Ouald Azzouz, Morocco Department of Microbiology, Mohammedia Faculty of Sciences and Techniques, Morocco Hygiene and Foodstuffs Industry of Animal Origin (HIDAOA), IAV Route Alal lfassi Al Erfane, Morocco Laboratory of Microbiology, Food, Products and Environment, Pasteur Institute of Morocco, MoroccoFulltext PDF
Objectives: Salmonella serotype Enteritidis remains the major causative agent of foodborne diseases and human infections worldwide. The purpose of this study was to investigate the molecular characteristics of nalidixic acid resistant Salmonella enteritidis in Morocco. Methods: A total of 862 human and nonhuman Salmonella strains were collected and isolated from 2002 to 2018 in different cities in Morocco. Antimicrobial susceptibility was determined using the disc diffusion assay and sequencing the PCR amplified gyrA and parC genes. Results: The overall percentage of Salmonella enteritidis was 12% (100/862). Drug susceptibility assay revealed that 80% of Salmonella enteritidis isolates were resistant to nalidixic acid. Our data further show that this resistance was due to mutations in gyrA (Aspartate (D) 87 Asparagine (N)) and in parC (Threonine (T) 57 Serine (S)) genes. PCR amplification and plasmid profiling analysis revealed that 80% of strains harbored the spvC virulence gene (669 bp) and a 54 kb plasmid. Conclusion: Our study showed a high rate of nalidixic acid resistant Salmonella enteritidis in human and nonhuman isolates in Morocco. Taking this into account is essential in order to take rapid measures in livestock to ensure control of this emergence of multi-resistant bacteria.
Salmonella enteritidis; Quinolone; sequencing; spvC
En-nassiri H, Hammoumi A, Berradi T, Rajaallah M, Essoucratti K, Asmai R, et al. Genotypic Characterization of Nalidixic Acid Resistant Salmonella enteritidis Isolated from Human and Nonhuman Origin in Morocco. Am J Clin Microbiol Antimicrob. 2019; 2(3): 1040..