Am J Clin Microbiol Antimicrob | Volume 1, Issue 5 | Review Article | Open Access

Epidemiological Study on Rift Valley Fever Virus among Humans in Taiz Governorate (Yemen)

Mansour Shayif AA^1*, Mohammed FA¹, Jamil SM², Saeed MA¹ and Ahmed MA³

¹Department of Microbiology, Sana'a University, Yemen
²Department of Biology, Sana'a University, Yemen
³Department of Laboratories, Hodeida University, Yemen

^*Correspondance to: Mansour Shayif AA 

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Abstract

Rift Valley Fever Virus (RVFV) is the etiologic agent of Rift Valley Fever (RVF), transmitted to humans by mosquitoes or direct contact with infected animals. This study was performed to preparing RVF antigen from liver of infected lamb to be used in detecting anti-RVFV antibodies by using Enzyme Linked Immunosorbent Assay (ELISA) and to study epidemiological prevalence of anti-RVFV IgM and IgG antibodies among humans which conducted in the Researches Center of Taiz University and Central Health Laboratory, Taiz Governorate, Southwestern Yemen. RVFV antigen was successfully prepared at a titer dilution of 1:3,200 by Chessboard (CB) ELISA assay. However, out of 234 samples, 154 samples were positive for ELISA assay, of which 49 (31.8%) were positive for anti-RVFV IgM and 105 (68.2%) for anti-IgG antibodies. The antigen was successfully produced, and successfully adhered to ELISA plates. Simple and inexpensive methods give good results. The result can be used to develop and refine predictive database for RVF transmission based on environmental and remote sensing data.

Keywords:

Rift valley fever; Antigen; ELISA; Epidemiology; Yemen

Citation:

Mansour Shayif AA, Mohammed FA, Jamil SM, Saeed MA, Ahmed MA. Epidemiological Study on Rift Valley Fever Virus among Humans in Taiz Governorate (Yemen). Am J Clin Microbiol Antimicrob. 2018; 1(6): 1028.