Am J Clin Microbiol Antimicrob | Volume 1, Issue 2 | Research Article | Open Access

Muscarinic Acetylcholine Receptor Agonists and Antagonists Regulate Yeast to Hyphal form Transition in Candida albicans

Awad Ali, Archana Wakharde and Sankunny Mohan Karuppayil*

School of Life Sciences, SRTM University, India

*Correspondance to: Sankunny Mohan Karuppayil 

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Abstract

Reversible transition from yeast to hypha in Candida albicans represents a good model system for studying cell differentiation. In this study, we have studied the effect of human muscarinic receptor antagonists, dicyclomine, solifenacin and flavoxate on C. albicans morphogenesis induced by muscarinic receptor agonists, acetylcholine and bethanechol. We show that muscarinic receptors antagonists inhibited yeast to hyphal form transition in a concentration dependent manner. Dicyclomine inhibited yeast to hyphal form transition induced by bethanechol and acetylcholine more than solifenacin and flavoxate. We are reporting that solifenacin and flavoxate have good binding with C. albicans Rrp9. Solifenacin and flavoxate formed hydrogen bond interactions with the residues GLU509 and ILE510 respectively in the active site region of Rrp9. Acetylcholine and bethanechol also bound with Rrp9 by forming hydrogen bond interactions. Acetylcholine formed hydrogen bond interaction with SER314 and SER323 while bethanechol formed hydrogen bond interaction with ASP384 and ASP506 at the active site of Rrp9. C. albicans Rrp9 may have a role in yeast to hyphal form morphogenesis and that muscarinic acetylcholine receptor antagonists may be repositioned as anti-virulence agents in C. albicans.

Keywords:

Agonists; Antagonists; Muscarinic acetylcholine receptor; Morphogenesis; Molecular docking; Rrp9 protein

Citation:

Ali A, Wakharde A, Karuppayil SM. Muscarinic Acetylcholine Receptor Agonists and Antagonists Regulate Yeast to Hyphal form Transition in Candida albicans. Am J Clin Microbiol Antimicrob. 2018; 1(2): 1008.

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